Plasmid diversity in neisseriae.

Transposition of Tn1545-delta 3 in the pathogenic Neisseriae: a genetic tool for mutagenesis.

The ability to study the virulence of pathogenic Neisseria spp. has been greatly limited by the absence of genetic tools which allow the construction of defined mutants. We have engineered a transposon system which allows random mutagenesis of the Neisseria genome at relatively high frequency. Tn1545-delta 3 is a 3.4-kb derivative of the gram-positive transposon Tn1545 encoding resistance to ka...

Molecular diversity of the plasmid genotypes among Rhizobium gene pools of sesbanias from different habitats of a semi-arid region (Delhi).

Plasmid genotypes of root nodulating rhizobial isolates of Sesbania, sampled from six ecologically distinct habitats, were characterized. Plasmid profile analysis revealed nine different plasmid types having molecular masses ranging from 30 to 300 MDa, distributed among six profile types that grouped the isolates into six plasmid classes. The six plasmid profiles were diverged from each other a...

Isolation of a high molecular weight polyphosphate from Neisseria gonorrhoeae

Neisseria gonorrhoeae, as well as other Neisseriae, produce polyphosphate. This polyphosphate exists in two forms. Approximately half of it is loosely associated with the cells and can be recovered by washing in neutral buffers under conditions in which no significant lysis of the cells is observed. The other half is either intracellular or tightly associated, because it requires digestion of t...

Gonococcal, but Not Meningococcal, Porin Pathogenic Neisseriae is Influenced by Factor H Binding and Function in Sialylated

Slide agglutination technique for the rapid differentiation of Mima polymorpha and Herellea from the Neisseriae.

The genera Mima and Herellea, members of the tribe Mimeae (De Bord, 1942), contain organisms which morphologically resemble the Neisseriae upon primary isolation. Since they are being isolated in increasing numbers from the spinal fluid of patients with acute meningitis, a method for their differentiation from the Neisseriae would be advantageous. Schaub and Hauber (1948) classified a gramnegat...